Description
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-B bound in the initial step. The color development is stopped and the intensity of the color is measured